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HRV Biomarkers

Heart rate variability as an objective window into autonomic nervous system function

What is Heart Rate Variability?

Heart rate variability (HRV) measures the variation in time between consecutive heartbeats. Contrary to what might seem intuitive, a healthy heart does not beat with metronomic regularity but rather shows constant subtle variation in response to autonomic nervous system input.

Key Measures

  • RMSSD: Root mean square of successive differences - reflects parasympathetic activity
  • pNN50: Percentage of successive RR intervals that differ by >50ms
  • LF/HF Ratio: Balance between sympathetic and parasympathetic tone

HRV in PAIS

Reduced HRV has emerged as one of the most consistent objective findings in ME/CFS and Long COVID:

  • Lower resting HRV compared to healthy controls
  • Blunted HRV response to postural change
  • Reduced HRV during sleep (when it should be highest)
  • Correlation with symptom severity and PEM

Clinical Value: HRV provides an objective biomarker that can help differentiate PAIS from deconditioning and track treatment response over time.